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Nahum,Laila Alves; Pereira,Sérgio Luiz; Campos Fernandes,Flora Maria de; Russo Matioli,Sergio; Wajntal,Anita. |
Partial cytochrome b and 12S rDNA mitochondrial DNA sequences of eight representatives of the Ramphastidae family were analyzed. We applied the linearized tree method to identify sequences evolving at similar rates and estimated the divergence times among some of the taxa analyzed. After excluding Ramphastos tucanus and Capito dayi from our data set, the remaining taxa presented a constant rate of DNA substitution, and branch lengths could be re-estimated with a clock constraint using the maximum likelihood method. Branch lengths were calibrated assuming that Galliformes and Piciformes split around 100 million years ago (mya). Our results indicate that Ramphastinae, and probably Capitoninae, diverged from other Piciformes in the Late Cretaceous (~82 mya),... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Divergence times; K/T; Mass extinction; Cytochrome b; 12S rDNA; Paleogeography; Phylogeny. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400003 |
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Klossa-Kilia, E.; Kilias, G.; Sfenthourakis, S.. |
We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the... |
Tipo: Article / Letter to the editor |
Palavras-chave: Oniscidea; Greece; MtDNA; RLFPs; Genetic isolation; Ligidium; Isopods; 16S rDNA; 12S rDNA; 42.74. |
Ano: 2005 |
URL: http://www.repository.naturalis.nl/record/209650 |
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